Distribution And Postmortem Redistribution Of Ketamine In Rats

Objective1 .To study the distribution of ketamine in acute poisoned rats.2.To explore the postmortem redistribution of ketamine in rats and the influence of temperature to postmortem redistribution. Methods1.GC and GC/MS methods are used to analyze and detect the ketamine in rats.The samples of blood and tissues, to which internal standard object(SKF_525A) is added, are alkalified and extracted by ethyl acetate. The extracts are qualitated by GC/MS method and quantitated by internal standard and working curve methods.2.Distribution and postmortem redistribution study: The rats in experimental group are given intragastric administration of ketamine with a dose of 290 mg-kg^-1 ,45 minutes later, they are executed by hypoxia method and then they are preserved at temperatures of 24 ℃ and 4℃. Cardiac blood, peripheral blood, liver, lung, kidney, cardiac muscle and brain are taken out to detect ketamine at different times after death (0h,12h,24h,48h). Rats in control group are given intragastric administration of physiologic saline, blood and tissues are taken out correspondingly and used as blank check. Results1 .The concentration of ketamine is comparatively higher in tissues and lower in blood.The concentration has significant difference(P<0.01).The sequence of ketamine content in rats is as follow : kidney > brain> liver> lung > peripheral blood >cardiac blood.2. In 48 hour after rats death, the ketamine content is going up in cardiac blood , lung, liver with time prolonging at temperatures of 24 ℃ (P<0.05 ) , the changing trend of ketamine content is ascending first and then descending in kidney (P<0.05) and no significant change can be seen in peripheral blood, brain and peripheral muscle (P>0.05) .3.There is no significant difference be shown in the change of ketamine content in blood and tissues preservde at 4℃ (P>0.05 ) . Conclusion1 .Ketamine is generally distributed in blood and tissues of rats. Ketamine content in tissues is higher than that in blood.2.Ketamine has distribution and postmortem redistribution in rats.3. Temperature has significant influence on ketamine content in blood and tissues after death in rats, the content changes little at lower temperature.