Research On The Relevance Of Syndrome Of TCM Of Peptic Ulcer And Mitochondria Cytochrome Oxidase.

Objective: Traditional Chinese Medicine excels in macroscopic syndrome differentiation, which lays emphasis on the research of functions while being weak in microcosmic syndrome differentiation, especially lack of profound research on tissue structure of substance on sub-cellular level. Cytochrome oxidase is the marking enzyme on electron transporting respiratory chain of mitochondria, with high affinity on oxygen and can make the respiratory chain react with maximal velocity. In order to make further observation and assessment of the relation of spleen governing transportation, transformation and structure, function of mitochondria, under the guidance of the theory of relevance between spleen of TCM and mitochondria, we chose enzyme histiocytic chemistry method under electron microscope to study the relevance of syndrome of TCM of peptic ulcer and mitochondria cytochrome oxidase.Methods: Separately choosing 5 examples of the patients of peptic ulcer including syndrome of spleen qi deficiency, syndrome of heat in the stomach and normal group. Taking gastric mucous tissue through gastroscope examination, incubating tissues by the methods of cytochemical indication of cytochrome oxidase through improved methods of DAB-4HCL as substrate, making samples by routine electron microscope with non-staining, observing the mitochondria structure and the distribution of cytochrome oxidase through gastroscope examination, making image analysis by stereological methods so as to reflect completely the characteristic of cyto-chemistry reaction of different syndromes.Results: Cytochrome oxidase reactive drop in the active site in normal group lined up evenly along mitochondria inner membrane and crista, There are plenty of mitochondria in the syndrome of heat in the stomach group with cytochrome oxidase reactive drop deposited in the majority of integralmitochondria membrane and crista and a few broken mitochondria membrane and crista. It shows that not only the quantity of mitochondria decreased in the acid cell of patients in syndrome of spleen qi deficiency group, but also with obvious damage in mitochondria structure and abnormal mitochondria and dyeing absence of a little mitochondria cytochrome oxidase (KO. 05), the mitochondria area Am and perimeter Bm of acid cell of gastric mucosa decreased in the patients of syndrome of spleen qi deficiency group compared with the normal group (P(.O. 05), accordingly, mitochondria pyknosis and mitochondria membrane density 8 m value decreased (K0. 01) . Enzyme response area Ae value decreased(FKO. 01) , accordingly, positive reaction density Revalue of mitochondria cytochrome oxidase decreased (P\0. 01) , positive reaction perimeter Be value also decreased because discontinuation of enzyme reaction in mitochondria membrane (K0. 01) ..Mitochondria area Am and positive reactive mitochondria membrane length Be value in patients of syndrome of heat in the stomach group without significance compared with normal group (PX). 05) , enzyme response area Ae value decreased( K0.01 ) , positive reaction density Rm value decreased because discontinuation of enzyme reaction in mitochondria membrane (P\0. 01) , the Am, Bm, Ae, Be and Revalue in spleen qi deficiency group were all lowered compared with heat in the stomach group (K0. 01) .Conclusion: There are correlations between different syndromes of TCM of peptic ulcer and mitochondria cytochrome oxidase. The changes of quality and quantity of mitochondria and cytochrome oxidase in patients of spleen qi deficiency group led to insufficient energy and disfunction of spleen governing transportation and transformation and serial spleen deficiency syndrome. All of these were an important ultra-micro pathological basis of syndrome of TCM. The research confirmed further the theory of relevance of spleen of TCM and mitochondria and elucidated exactly the essence connotation of spleen governing transportation and transformation on sub-cellular level.