Effects Of CD8～+T-Lymphocytes On Pulmonary Vessels Inflammation In A Rat Model Of Chronic Bronchitis And Emphysema

Objective (1) To study the characteristics of CD8+T-lymphocytes in intra-acinar pulmonary arteries inflammation in a rat model of chronic bronchitis(CB) and emphysema. (2) To evaluate the effects of Erythromycin on CD8+T-lymphocytes in CB and emphysema.Methods 21 healthy male Wistar rats were divided into 3 groups randomly: normal control group (group A, n=7). CB and emphysema group (group B, n=7). EM-treatment group (group C, n=7). The rat model of CB and emphysema was established by intratracheal installation of lipopoly- saccharide(LPS) twice and daily exposure to cigarette smoking for 4 weeks. group C were administrated with EM for 4 weeks. After 4 weeks, arteries blood gas analysis of each rat had been made. Pathomorphological changes of the small airways, areas of alveoli and pulmonary arteries were observed , the morphometric characteristics of intra-acinar pulmonary muscular arteries were analyzed. the immunohistochemical expression of the numbers of CD8+ T-lymphocytes were measured in small airways and adventitia of intra-acinar pulmonary arteries and the percentage of arteries infiltrated by CD8+ T-lymphocytes was examined. Relationships between variables were assessed using the Pearson's correlation.Results (1) Compared with group A, the small airways in group B showed that the tissue and cells were injured and the inflammatory cells infiltrated the airway walls, and the average areas of alveolus increased(p<0.01,each), group B had higher neutrophils, lymphocytes,CD8+ T-lymphocytes in the adventitia of pulmonary muscular arteries and the...