Clinical And Pathological Diagnosis Study Of Pediatric Neuronal Intestinal Malformations

Objectives: By the way of discussing on clinical and pathological characteristics of Hirschsprung's disease (HD) and Hirschsprung allied diseases (HAD), evaluating role of two specific reagents(neuron-specific-enolase, soluble-protein 100),The purpose is to know HD and HAD deeply, to increase accurate rate of preoperative and postoperative pathologic diagnosis.Methods: Clinicopathologic data were reviewed from 120 cases of suspected HD patients. Pathologic diagnostic conclusions of HE divide into categorical group and doubtful group, immunohistochemistrical labeling were performed in 59 cases for NSE, S100 to diagnosis clearly and evaluate role of those specific reagents in discerning IGCs and nervous plexus. the data were analyzed relatively between clinic, HE and immunohistochemistrical labeling, to summarize clinical and pathomorphological characteristics. Specimens from proximal colon of different ages were divided into groups and measured by micrograph analytical system, to study the relationship between Age and maturity of IGCs.Results: (1)All patients had a history of constipation.(2) NSE immunoactivities only expressed in the nerve stanza cell and nerve fiber. S100 immunoactivities expressed in the nerve fibers, Schwann cells and perineural statelite cells.(3) Immunohistochemistrical staining could increase the accurate rate of pathologic diagnosis. (4) It's Significant that the age affected maturity of IGCs,...