Immune Intervention Effect Of B.adolescentis On Mice With Type 1 Diabetes Mellitus

Objective To observe the immune intervention effect of B.adolescentis on type 1 diabetes mellitus (nonobese diabetic, NOD) mice, and to approach preventive effect of administration Bifidobacterium to type 1 diabetes mellitus (nonobese diabetic, NOD) mice in earlier period.Methods 40 female NOD mice aged four weeks were divided into two groups. One group(n=20) was orally administered by B.adolescentis (0.25mg/gBM/d, prepared viable organism suspension 0.5ml), and the other (n=20) was given PBS by the same regimen. Mice aged four weeks were administered until 30 weeks of age. At the age of fifteen weeks, the NOD mice (n=6/group) were put to death and the degree of insulitis were observed, expression of Fas and FasL on islet of Langerhans in both groups of NOD mice were examined by immunohistochemical techniques. The incidence of diabetes was detected at age of thirty weeks, at the same time , to observe insulitis and the change of Fas/FasL and T cell subsets of spleen.Results Lower positive rates of type 1 diabetes mellitus and its onset was expressed and delated in experimental group(28.57%) than that in control group(78.57%)(P< 0.05), At the age of 15 weeks and 30 weeks, the level of insulitis were lessen than control group , but the number of survival areas of Langerhans were higher than those of control group;At the age of 15 weeks and 30 weeks, expression of Fas/FasL in experiment group was weaker markedly than that of control group (P<0.01) . 30 weeks of age, CD₄⁺ CD₈⁺T cell subsets of spleen were lower obviously than control group. (CD₄⁺ T cell P<0.01, CD₈⁺T cell P<0.05) .Conclusion Oral administration B.adolescentis in earlier period could, reduce and delay the disease incidence of type 1 diabetes mellitus and abatement insulitis. The mechanisms of immune intervention has relationship with inhibited Fas/FasL system-mediated β cell apoptosis, and accommodated CD₄⁺ CD₈⁺T cell subsets,...