A Study On The Recombinant Antigens Of Multiple B Cell Epitopes Of Schistosoma Japonicum

Schistosomiasis is the second major parasitic disease of the world after malaria, affecting 200 million people. Diagnosis is the center in the control of schistosomiasis. The parasitological diagnosis, i.e. stool examination is insensitivity through implementation of coordinated control measures in China. Since immunodiagnostic assays are sensitive and specific, it has played a great role on epidemiological survey, assessment of control efficacy and determination of target population for chemotherapy in the endemic areas in China.The most of immunodiagnostic assays of schistosomiasis, which have been applied in China so far are using crude egg antigens or adult worm antigens. It is urgent to develop the more purified, more specific and more effective diagnostic antigens for instead of the crude antigens. In this study, we try to use bioinformatics technique combined with molecular biology and immunology techniques to predict and screen the B cell epitopes from some antigen candidate, and to develop the novel recombinant antigens of multiple B cell epitopes for diagnosis of schistosomiasis.