Expression And Purification Of Recombinant Human Interferon AlphaX In Escherichia Coli.

Interferons(IFNs) are a multigene family with the ability to induce antipioliferative, immunomodulatory, and potent antiviral activities. Interferons are divided into two groups designated type I and type II.IFN-γ is the only type II interferon, whereas the type I IFNs consist of four major classes: IFN-a, IFN-β, IFN-ω , and IFN-ι. IFNs are mainly used to treat various viral infections, and some tumor, such as hairy cell leukemia, AIDS-related Kaposi's sarcoma, chronic myeloid leukemia, and renal cell carcinoma.Type I IFNs have remarkable ability of antiviral and antiproliferative activities. They can be induced by viral infection, and have great similarity in amino sequences. IFNsa are multigene productions. They include 23 subtypes or more, and are produced by B lymphocyte and macrophage. IFNaX is one subtype of alpha interferons. Its mature peptide consists of 166 amino acid residues without carbohydrate. It was reported that its inhibition to virus is 30 times higher than that of IFNa1.In the past and present, many...