Autogenous Transplantation Of Bone Mesenchymal Stem Cells Induced For Treatment Of Myocardiac Infarction

Objective: To induce the differentiation of rabbit bone mesenchymal stem cells (MSCs) into myogenic cells in vitro, and to investigate the transplanted MSCs' survival and differentiation in myocardiac infarction. Methods: MSCs from rabbit were abstracted, isolated, cultured. After MSCs were induced by 5-azacytidine, MSCs' differentiation was studied by immunohistochemistry. The model of acute myocardiac infarction was established by ligating animal left anterodecendant arteries (LAD) of 20 rabbits. These rabbits were randomly divided into two groups: the experiment one(n=10) and the control one(n=10). The induced MSCs were labeled with 4'6-dimidino-2-phenylindone (DAPI) and autogenously transplanted into myocardiac infarction in the experiment group. However, no-cells cultural liquid were transplanted into myocardiac infarction in the control group. The transplanted MSCs' survival and differentiation were studied 4 weeks after transplantation.Results: The grow of MSCs is adherence to wall. So most of Haematopoietic stem cells and erythrocyte were excluded through changing cultural liquid. MSCs grew rapidly. The MSCs induced by 5-azacytidine...