Study On Ultrasound Induced Apoptosis Of Leukemia Cell K562 Cultured In Vitro

Objective to observe the effect of Ultrasound on apoptosis of K562 cells. Methods human leukemia cell line( K562) cultured in vitro are treated by ultrasound at a frequency of 1.8MHz. Apoptosis cells were evaluated by light and fluorescence microscope and detected using the flow cytometry assay (FCM) . Results The percentage of apoptosis in cells exposure to 8mv × 5min and 8mv × 10min were 1. 1% and 2. 6% respectively, 10mv×5min and 10mv × 10min were 9.7% and 21.6% respectively. The percentage of apoptosis in cells exposure to 7mv×30sec at 1/2h, 1h, 3h, 5h posttreatment were 1. 9%, 3.6%, 5.7% , 7.7% respectively;Typical morphological changes of apoptosis such as apoptotic body were found under light and fluorescence microscope. Conclusion Ultrasound can induce apoptosis in vitro in human leukemia cell line K562.