Evaluation Of Sensitivity Of Antitumor Drugs In Human Bladder Cancer By MTT Assay In Vitro

Objective: To evaluate the sensitivity of antitumor drugs and their combination in superficial bladder cancer in different individuals. Methods: From April 2005 to November 2005, sensitivity and inhibiting rates of antitumor drugs and their combination of 59 superficial bladder cancer samples from different individuals were studied by means of MTT assay and primary tumor cell culture. Results: Of 59 superficial bladder cancer samples, sensitivity tests were successfully performed in 45 samples, and the others failed, the successful rate was 76.3%.Diversity of inhibiting rates of antitumor drugs was observed in samples from different individuals. Except MTX in stage of G1 and G3, no correlation has been found between the phenotype of sensitivity assay and the pathological grade and stage. Sensitivity and mean inhibiting rates of antitumor drugs in the primary group were notedly higher than that in the recurrent group.The combination of two therapy agents, which have different mechanisms or different cell-cycle targets, showed significantly more sensitivity and higer mean inhibiting rates than single one in superficial bladder cancer in different individuals.Conclusion: According the results of drug sensitivity test, the individual chemotherapy, the relative sensitive drugs and their combination should be designed and selected so as to increase treatment effect, decrease side effects to the least and decrease the tumor recurrence.