Preliminary Study Of The Effective Ingredient From Metabolism Products Of Metarhizium Anisopliae

Subject: Provide a new thoughtfulness for preliminary studying the source of swainsonine (SW) and validate SW in the metabolism products of Metarhizium anisopliae. At the same time this work can establish the foundation of drug value of Metarhizium anisopliae for more studying, and find out other effective ingredients in the metabolism products.Method:①Cultured transferring Metarhizium anisopliae on PDA in a stated time;②Fermentation cultured Metarhizium anisopliae on Czapek′s medium and collected the fermented broth;③Used gas chromatography(GC) to show the character and the amount of SW in fermented broth;④P retreated fermented broth with centrifugation separation,filter sterilization,concentration method and so on. Tracked active ingredient by TLC and extracted and separated by repeat extraction,chromatographic separation and vacuum sublimation method in the metabolism products of Metarhizium anisopliae.⑤Used GC to show the character of sublimation;⑥Appraised chemic configuration of extracted substance by gas chromatography and mass spectrometry (GC-MS).Result:①Metarhizium anisopliae growth all right by cultured transferring on PDA;②The Czapek′s medium is used in fermentation cultured which choose quality rate of glucose and peptone is 2:1, culture temperature is 26℃, and pH is 6.5;③The concentration of SW is 10.2131mg·L-1 in metabolism products by GC, which is consistent with foreign report is 9.0400 mg·L-1;④Grained some crude SW by vacuum sublimated and used GC to appraise the character. The result showed that the crude sample has SW, and the content is 44.94%,and that is said extracted 0.0038g in every litre of fermented broth by this method. In fact, the concentration of SW is 10.2131 mg·L-1 in metabolism products, so extraction rate is 37.21%.⑤We found out that the metabolism products have five monomers and seven unknow chemical compounds by GC-MS, which is 14-methyl-,methyl ester pentadecanoic acid,1-methyl-7-(1-methylethyl)phenanthrene,1,4-Di(4-methlpheny)buta-1,3-diene,A-Neoga-mmacer-22(29)-en-3-ol,acetate and Di(2– ethylhexyl ) phthalate.Brief summary: These results show that the metabolism products of Metarhizium anisopliae have SW, and we gain crude SW and five monomers and unknown seven chemical...