Studies On The Isolation, Purification And Their Activities Of Polypeptides From Human Placenta

This paper reports the research about the isolation, purification and their antithrombotic activities of polypeptides from human placenta.Four products, B1-3 ,B2-2,B3-1,B4-2, were obtained from human placenta by the technologies of homogenization, repeated freezing-solving extraction, ultrafiltration, anion-exchange chromatography and Sephadex G-25 gel chromatography. The purity of the four products is over 95% identified by RP-HPLC. The single band was given both B3-1 and B4-2 by SDS-PAGE and IEF with amolecular weight of 13774.7±350, 7468.4±500 and isoelectric point of 5.9 + 0.5, 4.8 + 0.5 respectively . The UV absorption spectrum of the four purified products were also identified. B2-2 and B3-1 show positive reaction in phenol-vitriol test.The primary pharmacological experiments indicated that the extracts of low relative molecular masses from human placenta have marked anticoagulant effect in vivo but little effect in vitro.