Study Of The Effect Of Ethyl Pyruvate On Gut Barrier Function In Mice With Severe Abdominal Infection

Objective To research the mucosa pathology , the expression of receptor of advanced glycation end products(RAGE) in the Intestinal mucosa epithelial cell, intestinal and serum maleic dialdehyde (MDA) level and intestinal myelopet -oxidase (MPO) level of the SD mice with critical abdominal cavity infection by replicating the SD mice critical abdominal cavity infection model. Studying the change of the intestinal mucosa pathology, the RAGE expression, intestinal and serum over-oxidant with the critical abdominal cavity infection and the cure of Ethyl Pyruvate (EP) for the critical abdominal cavity infection, to explore the injury mechanism of intestinal epithelial, to illustrate the expression and significance of RAGE in intestinal epithelial cells and to clarify effect of EP on gut barrier function in mice with severe abdominal infectionMethod A total of 36 SD mice was divided randomly into three groups (n=12 each): control group (laparotomy only), CLP group [cecal ligation plus puncture (CLP) was performed] and EP group (CLP plus the EP subcutaneous injection). The changes in intestinal mucosa pathology and RAGE immune-histochemistry were observed and the RAGE index was evaluated; And intestinal MDA, serum MPO and MDA levels were detected on postoperative 24h and 48h (n=6 each point of time)...