The Effect Of D-Amphetamine On Motor Recovery After Brain Ischemia And The Molecular Mechanism Research In Rats

Objective:Through dynamic observing the change of motor skill, we study the effects of D-amphetamine on motor recovery after middle cerebral artery occlusion in rats. To investigate the dynamic changes of synapse,apoptosis and the expression of growth-associated protein 43(GAP-43),microtubule-associated protein2 (MAP2),neurofilament protein (NF) and synaptophysin (SYP) was observed by electron microscope,The terminal deoxynucleotidyl-transferase-mediated dUDP-biotin nick end-labeling (TUNEL),reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical techniques, and explore the molecular mechanism of D-amphetamine in accelerating rehabilitation .Methods:Along with the application of intra-luminal technology of modified Koizumi, fishing-line was used to create the focal cerebral ischemia reperfusion model in Clean-Grade Sprague-Dawley rats, extract the line after occlusion 2h. The rats were randomly divided into three groups:...