| Propose: To study the effect of COX-inhibitor on differentiation and function of DC cultured in supernatants derived from gastric cancer line.Method:1.generation of supernatant from gastric cancer line HCG-7901 There were A,B,and C three groups. A received nothing as control group,B and C groups were respectively treated by indometacin and celecoxib.The supernatants of A,B and C groups were prepared from gastric cancer line HCG-7901 after a 24-h culture.2.The induction and biological features of DC derived from peripheral blood mononuclear cell of health adults.DC isolated from peripheral blood mononuclear cell of health adults were cultured and proliferated in vitro in complete medium supplemented with recombinant human granulocyte-macrophage colony stimulating factor(rhGM-CSF) and interleukin-4(rhIL-4). The... |
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