The Influence Of Different Dialysis Membranes To The Apoptosis Of Peripheral Blood T Lymphocyte Of Hemodialysis Of End Stage Renal Disease Patients

Objective To investigate the apoptosis of T lymphocyte ,the expression of Annexin V and Bcl-2,Fas on the peripheral blood T lymphoyte in end stage renal disease(ESRD)patients;the characterization of Th1 /Th2 profile and the correlation with the apoptosis of the peripheral blood T lymphocytel;the influence of different dialysis membranes to the apoptosis of T lymphocyte of maintenance hemodialysis(MHD)patients.Methods The apoptosis of T lymphocyte,expression of Annexin V,Bcl-2,Fas on peripheral blood T lymphocyte cultured with Phytohemagglutinin (PHA) stimulated 24 hours were measured by Terminal deoxynucleotidyl transferase-mediated nick end labelling(Tunel),flow cytometry and immunohistochemical,enzyme linked immune sorbent assay (ELISA) was performed for detecting the level of IFN-γ,IL-4 in culture supernatants,radiate-immune assay was performed for detecting the serum beta2-microglobulin in 10 non-dialyszed(ND)patients,45 maintenance hemodialysis patients with cellulose acetate(CA)...