| Objective:To investigate the protective effects of SEA0400 (2-[4-[(2,5- difluorophenyl)methoxy]-5-ethoxyaniline), a newly synthesized Na+/Ca2+ exchanger inhibitor ,on ischemia/reperfusion cardiomyocytes of isolated rat heart.Methods:Isolated cardiomyocytes were obtained from adult rat hearts by Langendorff perfusion and enzymatic dissociation, and randomly divided into three groups including normal group, ischemia/reperfusion injury group(I/R group) and SEA0400 intervention group. The normal group was incubated in normal condition for 60 minutes as a control group. The I/R group underwent ischemia for 30 minutes and reperfusion for 30 minutes respectively. SEA0400 intervention group included 3 subgroups with SEA0400 at different concentrations(0.1μmol/L,1μmol/L,10μmol/L), and then underwent ischemia for 30 minutes and reperfusion for 30 minutes. MTT methold was applied for the measurement of the cardiomyocyte viability at the end of reperfusion period. The intracellular Ca2+... |
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