Effects Of Aloe Extracts On Rats Deep Partial Burn Wound Healing

Objective This study is to investigate the effects of gel and raw polysaccharide extracted from Aloe Barbadensis on levels of burn wound tissue microvascular formation, VEGF levels, wound healing, water contents, NO, ET and inflammation factors TNF-a, IL-1, IL-8 levels in deep partial burn wound rats . And to discuss the possible action mechanism of aloe extracts on wound.Methods 48 male Wistar rats were randomly divided into 2 groups: normal control group (n=6) and test groups (n=42), the test groups were randomly subdivided into 7 groups (n=6). 4 deep partial thickness wounds of diameter 3 cm were engendered on the back of every rat. Each wound respectively treated with once-daily application of 5% aloe raw polysaccharide, 10% aloe vera gel, 1% SD-Ag, normal saline. Wound tissues water contents,N0 ,ET , VEGF, TNF-a, IL-1, IL-8 levels ,wounds healing rate were detected at post burn 4h, 12h, 24h, 48h, 7d, 14d and 21d, NO/ET ratio were accounted, and microvascular density with factorâ…§immunohistochemistry staining were determined at the above time points .Results 5% Aloe raw polysaccharide and 10% aloe vera gel groups could visibly promote wound healing up , the wound healing rate were distinguished larger than the SD-Ag and normal saline groups(P < 0. 05)after post burn 14 days ; At post burn 12h, 24h, 48h, wound tissue water contents of 5% aloe raw polysaccharide and 10% aloe vera gel groups were less significantly than SD-Ag group(P < 0. 05), N0 and NO/ET ratio levels were lower than SD-Ag and normal saline groups(P < 0. 05),...