The Preliminary Study Of Composite Made By Recombinant Human Bone Morphogenetic Protein-2/human Transforming Growth Factor-β₁ Delivered In Collagen-I

Objective To synthetize rhBMP-2/hTGF-β1/collagen composite by recombinant human bone morphogenetic protein-2 ( rhBMP-2 ) and human transforming growth factors-beta1 ( hTGF-β1 ) and Collagen-I, to detect biocompatibility and activity. of the rhBMP-2/hTGF-β1/collagen composite.Methods With collgen to compound rhBMP-2 and hTGF-β1 make the rhBMP-2/hTGF-β1/collagen composite, to detect biocompatibility of the rhBMP-2/hTGF-β1/collagen composite with lagophthalmos conjunctiva stimulation test and hemolysis test, to detect activity of the rhBMP-2/hTGF-β1/collagen composite with tetrazolium salts (MTT) shade selection test and alkaline phosphatase (ALP) activity detection test and calcification nodus observing effect to Beagle marrow stroma cell (MSC) in growth and proliferative by composite.Results The rhBMP-2/hTGF-β1/collagen composite look like white flocc .or bolus. The lagophthalmos conjunctiva have no stimulus response after normal sodium soak of the rhBMP-2/hTGF-β1/collagen composite continuous contact lagophthalmos conjunctiva for 14 day, lagophthalmos bulbar conjunctiva have no inflammatory cell in optical microscope ; hemolysis test indicate haematolysis rate of the rhBMP-2/hTGF-β1/collagen composite to cony blood is -1.639%,which is lower than standard value 5%.The data of tetrazolium salts (MTT) shade selection test and alkaline phosphatase (ALP) activity detection test is undertaked analysis of variance...