The Study On Common Fragile Gene FHIT And WWOX Expression In Human Breast Carcinoma

Breast cancer is the most common carcinoma in western countries and many cities of China. The incidence of this disease has continued to rise. One of the aim in cancer research is to define molecular mechanisms leading to the initiation and progression of tumor cells. With the progress in understanding the biology properties of breast cancer, it is now widely accepted that breast cancer is a systemic disease initiate from changes on the gene level.In 1996,Ohta et al. identified a candidate tumor suppressor gene, FHIT, at 3p14.2, by exon trapping experiment.FHIT is classified into the gene family HIT(histindine triad),and as the first common fragile gene to be found,it is easy to rupture. FHIT gene covers a genomic region greater than 1Mb ,and it is situated in the fragile site FRA3B.In 2000, Bednarek et al cloned the WWOX gene with the shotgun technique, which spans a genomic region greater than 1 Mb in size and is the second most common chromosomal fragile site, FRA16D (16q23) about 2 Mb genomic region.FHIT and WWOX, as the two earliest discovered fragile site genes, play a very important role in the gene family of suppressor genes, and they are closely connected with the initiation and progression of the tumors, also with the prognosis of patients. In the present study, we investigated 1 .Expression of the fragile genes FHIT and WWOX mRNA in human breast cancer by using reverse transcription-polymerase chain reaction(RT-PCR) methods. In addition, whether expression levels of FHIT and WWOX are associated with specific clinicopathologic characteristics of .human breast carcinoma patients was determined. By multivariant analyses, we want to find out the risk factors...