The Relationships Of Matrix Metalloproteinase(MMP)-9, Its Inhibitors In Human Pulmonary Vein Blood With Lung Injury Following Cardiopulmonary Bypass

Objective To evaluate the relationships of matrix metalloproteinase(MMP)- 9 ,its inhibitors in human pulmonary vein blood with acute lung injury(ALI) following cardiopulmonary bypass(CPB).Methods Total 25 patients who have been performed the heart surgery were chosen in our study,and among them, 20 patients underwent extracorporeal circulation while while other 5 patients didn't. Blood samples were collected from the pulmonary vein at the commence of CPB (or of surgery) and at the termination of CPB(or of surgery) respectively, alveolar-to-arterial PO2 difference (A-aDO2) and respiratory index(RI) were measured at the same timethe. Enzyme-linked immunosorbent assay(ELISA) and reverse transcription-polymerase chain reaction(RT-PCR) were used for thedetection of MMP-9 and tissue inhabitor of metalloproteinase-l(TIMP-l); RT-PCR and western blot analysis for the detection of α2 macroglobulin (α2M) and soluble α2 macroglobulin receptor (Sα2MR) .The paired /-tests was employed to define the change of them , and Pearson correlation analysis was used to define the relationships of the changes of MMP- 9 JIMP-1 ,α2M,Sα2MR ,A-aDO2 ,RI following CPB.Results 1.Following CPB , the concentration of MMP-9 was increased obviously comparing to the decrease of the concentration of TIMP-l;and the ratio of MMP-9/ TIMP-1 was increased; while in the surgery without CPB, the concentrations of MMP-9 , TIMP-1 and the ratio of MMP-9/ TIMP-1 did not change evidently. 2. After CPB the concentration of Sα2MR was significantly increased,while the concentration of...