Investigation Of The Therapeutic Effect Of CNTF On The Retinitis Pigmentosa Of Rd Mouse

Objective: CNTF can be found in wide range of central and peripheral nervous system, and play a key role on surviving of traumatic neuron and regeneration of axon. In this research, we want to observe the therapeutic effect of CNTF vitreous cavity injection on (retinal degeneration) rd mice retina, and to find the combined therapy basis for RP.Methods: In the first section, postnatal 10 days (P10) rd mice were randomized into three groups, CNTF treated group, sham-surgery group and blank control group. CNTF and PBS were injected into the vitreous of the treatment group and the sham-surgery group respectively on P10. After anesthesia, ERG of rd mice were examined on postnatal 14, 18, 22 and 30days, and then the mice were killed by overdosage anesthetic. Transmission electron microscope, light microscope, measurement of thickness of the retina outer nuclear layer and TUNEL assay were carried out.Results: 1. It was observed that the retina outer nuclear layer of the treatment group was thicker than that of the other two groups under light...