The Role Of JWA In The Apoptosis And Neoplastic Transformation Induced By MNNG In Human Bronchial Epithelial (HBE) Cells

Malignant tumors are one kind of the most serious diseases of human being. Environmental pollutions are thought to be the important factors which contribute to the occurrence of the malignant tumor. It has already been well documented that many genes are identified and confirmed to involve in celluler proliferation, differentiation and apoptosis in tumor's progress. Many alkylating agents have been confirmed as carcinogens which widespread existence in the environment. Alkylating agents induce tumor by attacking tumor suppresor gene such as p53, and activating cancer target gene such as RAS as well as affecting the processes of DNA synthesis associated molecular events. The alkylating agent N-methyl- N'-nitro-N-nitrosoguanidine (MNNG) is a typical carcinogen and extensively used in inducing animal tumor and cell malignant transformation assay.JWA gene was primarily cloned from human trachea-bronchial epithelial cells using mRNA differential display-reverse transcription polymerase chain reaction (DD-RT-PCR) technique by Zhou, et al in 1998 (NCBI, AF070523.1). Several cis-elements was found from the JWA proximal promoter region, such as heat shock response element (hRE), stress response element (SRE), TPA...