Immune Tolerance Induction Of Small Bowel Transplantation By IL-10 Combined With Tripterygium Wilfordii Modified Dendritic Cells In Rats

Objective: To study the effect of allograft immune tolerance induction by donor IL-10 combined with Tripterygium wilfordii modified dendritic cells (DCs) for providing theoretical and practical basis for anti-rejection of small bowel transplantation. Methods: DCs were isolated from rat long bone marrow and cultured in vitro, seven days after the culture, interleukin-10 (IL-10) and tripterygium glycosides (Tw) were applied for two more days. Cell morphologic changes and major histocompability complex (MHC-II), CD80, CD86, CD40 expression were observed in the following groups: A (control), B (50ng/ml IL-10), C (5μg/ml Tw), D (200ng/ml IL-10), E (20μg/ml Tw), F (50ng/ml IL-10+5μg/ml Tw), G (200ng/ml IL-10+20μg/ml Tw). The recipient rats were pretreated with donor modified DC or with donor not modified DC. Small bowel transplantation was performed and the survival time of small bowel allografts were observed. Results: DCs in group G showed a significantly lower expression of the costimulatory molecules on surface, which could inhibit DC maturation and immune response activity obviously. The survival time in the groups of control, not modified DC and IL-10 combined with Tripterygium wilfordii modified DC was (7.33±2.42) days, (8.33±2.94) days and (18.5±5.17) days respectively. Statistical analysis showed that there was no significant difference between not modified DC group and control group.