Generation And Biological Characteristics Of Dendritic Cells From Malignant Pleural Effusions Of Patients With Lung Cancer

Objective: To generate dendritic cells(DCs) in vitro from malignant pleural effusions(MPE) of patients with lung cancer and identify the morphology, phenotypes and the biological functions of DCs, and to explore the value of cultured DCs on immunotherapy of cancer.Methods: Precursory DCs were obtained by adherence after density gradient centrifugation from MPE of 15 patients with lung cancer, and cultured with interleukin-4(IL-4), granulocyte macrophage colony-stimulating factor(GM-CSF), and tumor necrosis factor-α(TNF-α), and DCs cultured from peripheral blood monouclear cells(PBMC) of 6 healthy volunteers as control. The morphological features were observed by invert optical microscope; The capacities of DCs to uptake antigens were detected by FITC-Dextran uptake test; Phenotypes of DCs were analyzed by flow cytometry(FCM); MTT was used for the allogeneic mixed lymphocyte reaction(MLR), and ELISA for the levels of IL-10 and IFN-γin supernatant of cultured DCs; To evaluate the effect of DCs on the lymphocytes of MPE, phenotypes of lymphocytes were analyzed by FCM; Cytotoxicity of lymphocytes were detected by MTT.Results: (1) DCs derived from MPE had the typical morphology. (2) On d9 of culture, DCs showed significantly high expression of CD80(87.2±14.8)%, CD86 (92.1±2.9)%, CD83(53.8±11.3)%, HLA-DR(93.4±3.1)% and CD1α(41.4±18.4)% than those of d6 on DCs(P<0.05), but there was no difference compared with DCs from...