The Effect Of Interferon Alpha-2b Combine With Cisplation On The Growth Of Hormone-refractary Prostatic Carcinoma Implants In Nude Mice

Objective: To investigate the effects and mechanism of Interferonalpha-2b Combine with Cisplation for the Growth of Hormone-refractary prostatic Carcinoma Implants in Nude Mice.Methods: Nude mice bearing human Hormone-refractory prostatic carcinoma (HRPC) were randomized into the following group 10 days after implantation of human prostate carcinoma cell PC-3 :group A ,saline,sc,qd (control);group B, IFNα-2b 10000IU/,sc,qd;group C criplation 1.5mg/kg/,ip,biw and group D IFNα-2b 10000IU/,sc,qd plus cisplation 1.5mg/kg/,ip,biw,respectively ;Total mice were sacrificed four weeks after administration. Then the volume and weight of tumor,the post-therapeutic weight of mice were studied after 28 consecutive days.The COX-2 and VEGF expression of tumors were detected by immunohistochemistry.Microvessel density (MVD) was counted by endothelial cells immunostained by anti-CD34 antibody.The TUNEL assay was used to examined the apoptosis alteration of tumor cell in nude mice after treatment with saline ,IFNα-2b , cisplatin and combination group.Result:In control group , IFNα-2b group , cisplation group and combined group ,the tumors weighted was 1.0570±0.18734g, 0.8562±0.12114g, 0.5784±0.10953g and 0.3322±0.13353g,respectively;the tumor inhibition rate was 0% ,18.99%,45.28% and 68.57%, respectively .the tumors volume was 1318.90±296.3mm3, 863.486±269.6 mm3, 474.24±104.28 mm3 and 157.50±62.310 mm3.The tumor growth of every therapeutic groups were significant different from the control .The IFNα-2b-treated tumors were efficiently smaller in volume and lower in weight than...