Expression Of Skp2 In Patients With Acute Leukemia And Its Relationships With P27(Kip1) And Ki67

Background and Objective: It is a common character that tumorigenesis is related to disfunction of cell cycle and endless proliferation of cells. The acute leukemias are clonal malignancies of hematopoietic cells that are characterized by the abnormal proliferation and inhibition of apoptosis. Many ongenes, tumor suppressor genes and their related genes disorder, which make cell cycle deregulated, cells proliferate excessively and the apoptosis of cells inhibitted, promoting leukemogenesis. The progression of cell cycle demands on precise restriction and proteolysis of cell cycle regulating factors, which is associated with Ubiquitin-proteasome pathway.Ubiquitin-proteasome pathway is one of the important system for regulating protein, which plays a pivotal role in controlling cell cycle progression, gene transcription and signal transduction through degrading the Ubiquitin-proteins. Skp2, a member of the substrate-recognition subunit of SKP1 Cullin F box(SCF) ubiquitin ligase complex, is required for the G1-S transition. It has been implicated in monitoring cell proliferation and apoptosis through hydrolyzing multiple target proteins.p27(Kip1), a cyclin dependent kinase inhibitor, is a suppressor of the G1/S checkpoint.In G0 cells,levels of p27(Kip1) are high, once cell cycle passes the Gl/S checkpoint, there have been the Thr-187 phosphorylation of p27(Kip1) and its subsequent ubiquitin-mediated degradation. High levels of p27(Kip1) suppress the process of cell cycle, restraining cells in G1, inhibiting cells proliferation. Skp2 is...