Nitrosylation Precedes Caspase-3 Activation And Translocation Of Apoptosis-inducing Factor In Rat Cerebral Chronic Stress

Stess is harmful to human body. many diseases are related with stress, such as depression, affective disorder. In the last few years, several reports indicated that long-lasting stress affects synaptic plasticity, dendritic morphology, and neurogenesis. chronic unprectable stress induce brain injury by some pathogenesis, especially damage is very seriously in cortex and hippocampus. One of the mechanisms of excessive NO production involved in the development of injury after chronic unprectable stress is that it combines rapidly with superoxide to form the powerful oxidizing agent peroxynitrite (ONOO~-).ObjectiveWe characterized the spatial and temporal formation of peroxynitrite through immunohistochemical detection of nitrosylated proteins. Nitrotyrosine staining co-localized with two other cellular markers of injury, active caspase-3 and nuclear translocation of apoptosis-inducing factor (AIF). Pharmacological inhibition of neuronal and inducible nitric oxide synthase (nNOS and iNOS) using Uric acid, which has been...