Observation On The Levels Of Anti-Trichinella Antibodies In Muscle Juice Samples Of Experimentally Infected Mice.

Trichinellosis is a serious parasitic zoonosis mainly caused by eating raw or undercooked pork and the meat of other animals containing Trichinella muscle larvae. The quarantine of Trichinella in meat and its products is regarded as an important task in for control of trichinellosis. At present, the quarantine methods of Trichinella in meat consist of trichinelloscopy and artificial digestion, but the sensitivity of the former is low, the operation of the later is more complicated to be performed. Since the IgG antibodies are distributed in both serum and muscle juice of host, immunological methods are expected for the detecting anti-Trichinella antibodies in muscle juice. However, the dynamic changes of anti-Trichinella antibodies in muscle juice samples of infected animals are not reported. So, a murine model will be used both to observe the dynamic changes of anti-Trichinella antibody levels in serum and muscle juice of mice infected with different dose of infection. In addition, the dynamic changes of anti-Trichinella antibodies in muscle juice of infected corpse reserved at different temperature and different time were observed by indirect ELISA using an excretory-secretory (ES)antigen of Trichinella spiralis muscle larvae..Materials and methods1. The best experimental conditions of ELISA for detecting antibodies in muscle juice of mice infected with T. spiralis were selected, such as antigen concentration, serum dilution, the kind of second antibody and the dilution of muscle juice samples.2. 300 mice divided into 3 groups randomly(100 mice per group) were inoculated with 100, 300 and 500 muscle larvae of T. spiralis, respectively. The anti-Trichinella antibodies in serum and muscle juice taken weekly up to 18 weeks post-inoculation (wpi) were evaluated.3. 32 mice divided into 4 groups freely (8 mice per group) were inoculated with 50,100,300...