Identification Of Osteogenesis Genes Responsible For Mechanical Stretch-induced Differentiation Of Human Periodontal Ligament Cells

The basic procedure of orthodontic treatment is tooth movement in a certain direction resulted from periodontal tissue biological responses and remodeling induced by exogenous mechanical forces. So, the mechanical force is the premise and the key to promote human periodontal ligament cells( hPDLCs) physiologic reaction and to result in orthodontic tooth movement. Previous studies indicated that mechanical stretch can induce osteogenesis differentiation of human periodontal ligament cells( hPDLCs).To investigate the effect of serum concentration on the biological activities of cultured human periodontal ligament cells (hPDLCs). Certain concentration of the fifth generation of cultured hPDLCs was seeded into 96 plates, to determine proliferation of the hPDLCs using MTT method. And use flow cytometer to determine the effect of serum concentration on the cell cycle of the hPDLCs. The proliferation activity and proliferation index of the hPDLCs are lowest in 2% serum concentration and highest in...