The Effects Of Nucleic Acids From Lactobacillus Fermented Filtrate On Anti-tumor And Immunological Function In Tumor-bearing Mice

Objective: Lactobacillus is one of the predominant microorganisms in human normal flora. A lot of research and practice has proved that it may stimulate host body immunity to maintain human health. Epidemiology investigation suggested that the habitual intake of milk products fermented by lactobacillus reduces the incidence rate of mammary cancer, colorectal cancer, stomach cancer and bladder cancer. But the molecular mechanism of its anti-tumor molecular effect is unknown. We founded a lot of nucleic acids existed in lactobacillus DM9811 fermented filtrate. Report proved the augmentation of T(H)-1 type immunologic reaction by AT oligonucleotide from lactic acid bacteria. It suggested the nucleic acids from lactobacillus is the important factor to augment cell immune response so that it has anti-tumor effect. It is the possible mechanism of lactobacillus anti-tumor effect that these lactobacillus extracelluar nucleic acids can up-regulate host immune responses level. This experiment observed the effects of nucleic acids from lactobacillus DM9811 fermented filtrate on tumor cell growth and immunological function in tumor-bearing mice, then studied its character, source, anti-tumor activity, possible molecule mechanism and investigated the possibility that it became new anti-tumor activity material.Methods: 1, Measured lactobacillus DM 9811 growth curve and chose the different time in log phase, fermented filtrate...