Extraction Of Anti-endotoxin Components From Terminaliachebula Retz And Their Biology Activity Evaluation

Objective:Lipopolysaccharides (LPS), major part of extracellular membrane of Gram-negative bacteria, is recognized as a key molecule in the pathophysiology of sepsis. LPS can bind to its receptors in cell membrane through its active region-lipid A ,and then induce sepsis. So blocking the bind of lipid A to the receptors is considered as the most promising strategy to prevent the initiation of sepsis. Clinical experiences and studies had suggested that many traditional Chinese medicines possess the function of anti-sepsis. since there are complicated chemical constitutes in the plants, the material basis of the anti-sepsis activity is not clear. Based on the biosensor by target of Lipid A, we screened the active components extreated from Terminaliachebula Retz, and studied their biological activities against sepsis.Methods:(1) The biosensor technology was applied to screen anti-inflammatory traditional Chinese medicines targeted lipid A (2) The active fractions were isolated by silica gel chromatography ,polyamide chromatography , ion-exchange HPLC.Among the obtained fraction of Terminaliachebula Retz, the one with the best affinity to Lipid A was chose to receive futher isolation,to observe the activity of binding to lipid A with biosensor. (3) In vitro, studies were carried out to investigate the LPS-neutralizing ability of this fraction ;and its inhibition of TNF-αrelease from RAW264.7 cells induced by LPS and CPG ODN. In vivo, the fraction was also be used to administer the mice after lethal challenge of LPS or hot-inactivated E coli.Results :(1) Among all the seventy-eight traditional Chinese medicines, there are twelve herbs having the higher binding activity to lipid A. the components in water extract from Terminaliachebula Retz was identified with a higher binding activity,so was selected to...