The Implication Of HPV DNA Test In Diagnosis And Follow-up Of Cervical Cancer And Its Precursors

Objectives: Cervical cancer is the second most common gynecological cancer in women following breast cancer. During the recent decade, the human papillomavirus (HPV) infection was found to be a necessary cause of cervical cancer, but not sufficient alone. High-risk HPV infections increase the risk of developing CIN and cervical cancer. But the usefulness of the HPV DNA test in diagnosis and follow-up of cervical cancer and its precursors is still not fully understood. The purposes of our research were to investigate the correlation between infection of high-risk HPV and the development of cervical cancer, to investigate the correlation between high-risk HPV DNA load and the severity of CIN and cervical cancer, and to assess the usefulness of HPV DNA test in diagnosis and follow-up of cervical cancer and its precursors.Methods: We collected clinical data and cervical exfoliated cells (vaginal stump exfoliated cells) from 514 patients (49 with cervicitis, 222 with CIN, 243 with cervical cancer) in Cancer Hospital before or after treatment. Hybrid Capture II was employed to detect high-risk HPV DNA. Viral load was measured by light measurements expressed as relative light units (RLU)/ cutoff (CO).Results: The positive rate of HPV DNA test in cervicitis, CIN and cervical cancer were 43.75%(21/48), 86.79%( 138/159), and 95.96%( 190/198), respectively. The median HPV DNA loads of cervicitis, CIN and cervical cancer were 121.5, 395.5 and 459.8, respectively. The viral load of CIN and cervical cancer was significantly...