Relationship Between VIP,SP And Diabetic Colonic Functional Disorder

Background: Diabetic colonic functional disorder is diabetes mellitus common chronic complication. Approximately 30-76% of patients with diabetes mellitus have gastrointestional complication such as diarrhoea and constipation. Diabetic colonic functional disorder affects the quality of life in diabetic patients. Recent data regarding pathogenesis are confusing unclear. The pathogenesis of diabetic colonic functional disorder is sometimes linked with autonomic neuropathy, gastrointestinal hormones, hyperglycemia, or a myogenic mechanism. Vasoactive intestinal peptide and substance P are neuropeptides that play important roles in regulating gastrointestional motility. This study will analyze the relationship between levels of vasoactive intestinal peptide,substance P in plasma and colon tissue and diabetic colonic functional disorder.Methods: 1. The rectal pressure, rectal visceral perception thresholds to balloon distension and the distribution of VIP and SP proteins in the colonic tissues in 54 cases of diabetes mellitus and 15 cases of normal controls were detected by Evision immunohistochemistry and perfusion catheter manometer. The slides were then analyzed with an Image Analyzing system to obtain the density(mean) of stained proteins. 2. The concentrations of VIP and SP in plasma were determined with radioimmunoassay.Results: 1. The rectal resting pressure, maximal squeezing pressure and defecation pressure in the patients between DM and control group were no difference (p>0.05). Thresholds of first perception, defecation and maximal tolerable in the patients with diabetic diarrhea were significantly lower than control group(p<0.01).Thresholds of first perception, defecation and maximal tolerable in the patients with diabetic constipation were significantly higher than control group(p<0.01). 2.Plasma VIP concentration in the normal controls, the diarrhea, the constipation and non gastrointestinal symptoms with diabetics were 46.29±15.47,27.59±17.73,26.39±13.09, 27.15±10.29;VIP levels in plasma were lower in diabetics than those of the controls(p<0.05).Respectively. SP concentration were...