The Distrition Of E. Faecalis On Infected Mice And Caused Histopathology Change

Enteroccoccus is human and animal's intestines constant bacterium. It's can cause cutaneous infection, soft tissue infection, celiac infection, blood poisoning, endocarditis and encephalitis.Infected white mouse through three ways: rhinal inoculation , caudal vein inoculation and celiac inoculation with five different dosages. The results showed that celiac inoculation is the best way to cause white mice infected and dead. The median lethal dose is 9.3billion.After infecting white rat with Enterococcus faecalis by celiac inocuation, Enterococcus facials could be separated from spleen and liver at 4 hours after hypodermic inoculation. 6 hours later, the bacterium could be separated from spleen, liver, and heart. The bacteria could be isolated from spleen, liver, heart, kidney and brain at 12 hours after inoculation. Some mice behaved obvious clinical symptom and dead. Dissect the dead mouse, obvious pathological change can be found, such as liver fatty degeneration , heart waxy necrosis, encephalitis, interstitial pneumonia .Isolated serum from infected white mice, detect antibody titer with precipitator method. Five days after celiac inoculation, antibody was produced, and two weeks later antibody titer reach the peak value and went down.