Raman Spectroscopic Studies Of Human Stomach Tissues And The Interaction Of Fibrinogen With Spermine

Raman spectroscopy is an important method for molecular structure analysis, which was discovered in about seventy years ago. Especially, Raman spectroscopy is advantageous and able to provide plenty of valuable information in biological and medical regions. Two partitions are involved in this thesis:Firstly, the interaction between Fibrinogen and Spermine and a tentative examination on the Spermine effects on the structure of Fibrinogen by Raman spectroscopy are focused for the first time. Spermine is a widely distributed and positively charged component in the living cells. In recent decades, several researches have found Spermine could precipitate the plasma. One of the main precipitates is the Fibrinogen, which is a protein rich in blood and plays an important role in platelet activation and aggregation. By comparing the spectra, it is concluded that the relative content of random-coil conformational structure of the Fibrinogen increased by adding the Spermine, and then the microenvironment and hydrogen bonding status of some amino acid residues changed. Additionally, the carboxyl groups of Fibrinogen are most likely to combine Spermine by static electrical force and lead to a diminishment of molecular repulsion between the proteins.Secondly, the Raman spectroscopy is used to explore the gastritis and gastric ulcer tissues. In recent decade, great progresses have been made on Raman spectroscopy technology applying in biological tissues. However, there is still a long way for Raman spectroscopy to be a practical diagnostic method. More experiments are needed for the purpose. In this part, differences between the both tissues were studied and a division by Raman spectroscopy was made. The differences are assigned to protein, lipids and nucleic acids respectively. By contrasted with gastric ulcer tissue, from the spectroscopy of gastritis tissue, it can be concluded that: 1) the contents of a-helix structural protein and the amino acids are relative reduced, the microenvironment of some amino acids are changed. 2) The stability and saturation of...