Influences Of Compressive Stress On The Expression Of Endothelial Lipase And Adhesion And Possible Molecular Mechanisms In Vascular Endothelial Cells

Objective: To investigate the effect and the possible mechanisms involved in the effects of compressive stress on endothelial lipase(EL) expression and adhesion of human umbilical vein endothelial cells line(HUVEC-12).Methods: HUVEC-12 were cultured under different static pressure at 120, 150, 180,210mmHg levels, respectively, in a self-manufactured pressure incubator for 24hrs or were treated with 180 mmHg of static pressure for 3, 6, 12, 24h. RT-PCR, western-blotting, flow cytometry and immunofluorescence were used to detect the expression of endothelial lipase(EL), inhibitor of nuclear factor-κB(IκBα) and NF-κB, respectively. The determination of adhesion rates between HUVEC-12 and THP-1 cells was performed by cell counting.Results: Compression stress treatment significantly induced the expression of EL mRNA and protein levels in a dose-dependent manner; with 180mmgh static pressure, EL mRNA and protein levels were increased about 1.58 and 2.54 times, respectively, at 24h compared with control. Compression stress induced the degradation of IκBα and the nuclear accumulation of NF-κB p65 by activating the IκB kinase(IKK). Static pressure...