The Initial Study Of Vaccine's Character About Irrad Iated Colon 26/IL-21 Cells And It's Antitumor Effects

Objective: Interleukin (IL)-21 was a rencently described type I cytokine which could kill some kinds of tumor cells by modulating B,T,and natural killer cell responses. Interleukin (IL)-21 had became a new sak gene in tumor therapy . Murine colon carcinoma cells(colon 26) that were retrovirally transduced with mouse IL-21 gene were inoculated in nude mice , the growth of colon 26/IL-21 tumors developed slowly.These data suggested that expression of IL-21 in tumors had antitumor effects. Colon 26/IL-21 cells were inoculated in syngenetic mice then colon 26/IL-21 tumors developed. In our experiment we want to culture the non-oncogenic colon 26/IL-21 cells vaccine by irradiating at isotope Co⁶⁰ 50Gy, study the vaccine's character non-Oncogenic of colon 26/IL-21 cells contrast with colon 26/IL-21 cells and it's antitumor effects.So we could provide the base theory in gene therapy of human colon carcinoma.Methods: 1 Murine colon carcinoma cells(colon 26) that were retrovirally transduced with mouse IL-21 gene were irradiated at isotope Co⁶⁰ 50Gy,so we obtained the vaccine of irradiated colon 26/IL-21 cells. Vaccine of irradiated colon 26/IL-21 cells were observed morphological features at light...