Antitumor Activity Of YB-13 And Its Mechanism

D-24851 is a novel synthetic compound that was identified in a cell-based screening assay to discover cytotoxic drugs. D-24851 destabilizes microtubules and blocks cell cycle transition specifically at G2-M phase. And it has undergoing phase I clinical trial. A serious of novel derivatives of D-24851 were synthesized by Department of Pharmacy of Medical College of Chinese People's Armed Police Forces. MTT assay showed many compounds had notable cytotoxic activities against human cervica cancer cell line in vitro. Further more, we select one of these compounds YB-13 to study the cytotoxic activity on kinds of cultured human cancer cell lines in vitro, inhibition on the growth of transplantable tumors in mice. Meanwhile we have investigated the mechanism and characteristic of the cytotoxic activity of the YB-13.1 Effects of YB-13 on the proliferation of cancer cellsIn a series of experiments, five tumorigenic human cells, including nasopharyngeal carcinoma cell human lung adenocacinoma cell (A549), human cervical cancer cell (HELA), human ovarian carcinoma cell (SKOV3), and human stomach carcinoma cell (BGC-823) were chosen to determine the cytotoxic activity of YB-13. After incubating for 96h, YB-13 led to a gradual decrease of viable cells fraction with increasing...