The Morphological Study Of Gastrointestinal Enterochromaffin Cells And Mast Cells In Rats With Cathartic Colon

Objective: This experiment established a rat's cathartic colon model using rhubarb. In the process of model preparing, we observed the population and morphous of enterochromaffin cells and mast cells and judged the metergasis of them. When model constructed, we detected the content of 5-HT in ho-mogenate of duodenum and sigmoid colon and in serum, we studied the effect of long-term application of rhubarb on the synthesis and release of 5-HT in gastrointestinal tract and inves-tigated the functions of enterochromaffin cells and mast cells in the development of cathartic colon.Method:Seventy two clean healthy adult Wister rats, half female and half male respectively, were chosen and divided randomly into control group and rhubarb group. Marked weight and sex difference do not exist after grouping.Leachate of rhubarb was intragastric administration in rhubarb group. At the beginning, the dosage is 800mg/kg.day (raw material), afterwards the dosage increased 200mg/kg.day (raw material)every day until half of rats'stool became watery, this dosage was maintained until 80 percent of rats'watery stool disappeared, then the dosage continually increased until 50 per-...