Experimental Study Of Biocompatibility Of Calcium Alginate Film And Osteoblasts From Human's Marrow

Objective:To investigate hunman bone marrow mesenchymal stem cells(MSC) in vitro through isolation,cultivation of MSC, and differentiate into osteoblasts. TO observe MSC on biocompatibility of Calcium alginate film.Methods:MSC was isolated and cultured in vitro and differentiate into osteoblast. The osteoblasts were cultured together with CAF in vitro. The proliferation and differentiation of MSC were examined by phase-contrast microscope, MTT, alkaline phosphatase(ALP) staining and the determination of ALP activities. There were two groups in the experiment which to study the effects of osteoblast on biocompatibility of Calcium alginate film. Induced group: The osteoblasts were cultured together with CAF in vitro.Control group: The osteoblasts were cultured individually.Results:MSC proliferated,and adhered to the plastic surface and like long spindle-shape fibroblastic cells.The human bone marrow stromal cells cultured in primary and passage culture were proliferated actively.After HBMSC's inducement for 2 to 3 weeks,these differentiated cells survived well. CD166 expressed positive,and CD34 negative by flow cytometry. The human osteoblasts derived from bone grow as well on CAF as normal cultured cells.These osteoblasts similar to the typical osteoblasts proliferated quickly,and demonstracted morphologic characteristics and biological properties and CAF dose no harm to their functions.Conclusion: HBMSC can be aspirated from bone convenienty, and they can be induced to osteoblasts differentiation possessing atypical morphologic characteristics and biological function in a defined condition, thus HBMSC can be used as ideal resource of seeding cells for bone tissue engineering. CAF processes a porous structure, which contributes to attachment, growth of osteoblasts, but dose no harm to their functions.The CAF has good biocompatibility with osteoblasts.