The Clinical Value And Levels Between Eotaxin, Eosinophils In The Induced Sputum And Immunoglobulin, T-cell Subgroup In The Blood Of Childhood Asthma

Objective: To explore the mechanisms of asthma by compared the levels between Eotaxin, Eosinophils in the induced sputum and immunoglobulin, T-cell subgroup in the blood of childhood asthma in order to provide new evidence for clinical diagnose and therapy.Methods: Sputum supernatants of 15 patients with asthma outbreak, 15 patients recovered and 10 volunteers as control were analyzed. Sputum inductions were performed by inhalation of hypertonic saline solution (4% NaCl). Eosinophils were counted on each slide. Eotaxin were measured in sputum supernatant using enzyme-lined immunosorbent assay. T cell subgroup in the blood was assessed by flow cytometer. Immunoglobulin was measured by single immune diffusion test.Results : 1 Significantly (P<0.05)increased levels of Eotaxin had been measured in induced sputum of asthma outbreak group compared with recovered group and control group. Moreover, no significant differences(P>0.05) were observed between recovered group and control group.There were significant difference(sP<0.05) between asthma outbreak group and control group of the Eosinophils in the sputum slides but no significant differences (P>0.05)compared asthma outbreak group to recovered group. There were significant differences(P<0.05) between asthma recovered group and control group of the Eosinophils in the sputum slides. Eotaxin was positively correlated with Eosinophils(r= 0.802, P<0.001). 2. There were significant differences(P<0.05 )between asthma outbreak group, recovered group and control group for the CD3+,CD4+,CD4+/ CD 8+ value in the blood. Significant difference(sP<0.05) of CD 8+ were observed between asthma outbreak group and control group. 3 There were significant differences(P<0.05)of IgA compared control group to recovered groups and asthma outbreak group respectively, but no significant differences (P>0.05)between asthma outbreak group and recovered group. Compared asthma outbreak group to recovered group and control group respectively, significant differences(P<0.05)of IgG were observed. There were significant differences(P<0.05)of IgE between 3 groups.Conclusion: Both Eotaxin and Eosinophils are involved in the mechanism of asthma. Eotaxin and Eosinophils in the induced sputum can reflect the degree of...