| Irritable bowel syndrome (IBS) is a functional bowel disorder characterized by visceral hypersensitivity and altered bowel motility. Recent studies indicated that the pathogeny of IBS was complex and involved with multisysterm. Due to be short of ideal therapy and drug, symptomatic treatment is the primary treatment at present. Selective 5-hydroxytryptamine receptor conditioners could improve some symptoms of IBS, but were limited in clinic because of their side effects. Multi-target, integrity effect and few side effects are superiority of traditional Chinese medicine (TCM).The clinical reports showed that curative effect of TCM was superior to that of Western medicine. Stagnation of liver-energy and asthenia of spleen was one of the most common types and was treated by the therapy of comfortable liver strengthen spleen. The Shugan Jianpi (SJ) Decoction is frequently used in PiWei institute to treat IBS.To investigate the effect and mechanism of SJ Decoction, the model of chronic visceral hypersensitivity was established which adapted to the study of traditional Chinese medicine. The investigation could not only provide experimental proof of guiding clinical medication and developing new medicine, but also conduce to study the treatment of IBS with TCM. 1 Replicate the model of IBSIn order to replicate the model of chronic visceral hypersensitivity in adult rats, SD rats of the model group were given acetic acid in rectum every day between postnatal 8d and 21d. Rats of the normal group were given normal saline. Then the threshold of abdominal withdrawal reflex (AWR) was measured at 6w and 8w with rectal distension and the amplification of movement in isolated rat' intestine caused by Ach or 5-HT was tested. The threshold of abdomen lifting and body arching of the model group showed a obvious decrease compared with the normal group(P<0.01). The amplification of movement in isolated intestine of model rat stimulated by Ach or 5-HT was greater than that of normal group (P<0.01). Chronic colon irritation in neonatal rats leaded to a state of chronic visceral hypersensitivity toadult rats. The isolated intestine of the model rats had high responsiveness.2 Effect of SJ decoction on the model of IBSFive litters of neonate rats were divided into five groups at random, followed by normal group, model group, SJ decoction low dose (SJL) group, SJ decoction high dose (SJH) group, Zelmac group .In order to replicate a model of chronic visceral hypersensitivity, all the rats between postnatal 8d and 21 d were given acetic acid in rectum every day except the normal group which was given saline in rectum every day. Then the threshold of AWR was measured at 6w and 8w during rectal distension. The threshold of abdomen lifting and body arching of normal group showed an obvious increase compared with the other four groups (PO.01). Then the rats were treated with administration of distilled water 25ml/kg, NS 25ml/kg, SJ decoction 5g/kg, SJ decoction lOg/kg, Zelmac 4mg/kg from 9w to lOw. After the treatment the threshold of AWR was measured again. Then the quantity of mast cell of colon was weasured. The content of NO in blood , the content of 5-HT in bloods brain and colon were observed in the experiment. The results showed that the threshold of AWR of SJL and SJH group showed an obvious increase compared with the model group (PO.01, PO.01). The quantity of mast cell of colon of SJH group showed an obvious decrease compared with the model group (P<0.05). The content of NO in blood of SJL and SJH group showed an obvious increase compared with the model group (P<0.05, PO.01). The content of 5-HT in blood of SJH group showed an obvious decrease compared with the model group (P<0.01). Experiment study confirmed: With the effects of S J decoction ,the threshold of AWR and the content of NO in blood could be increased , the quantity of mast cell of colon and the content of 5-HT in blood could be decreased, the content of 5-HT in brain and colon could be decreased to a degree. The SJ decoction showed affirmative curative effect on treating rats of IBS. It could be one of the mechanisms of action to influence the content of NO and 5-HT.3 Effect of SJ decoction on calnK analgesic > antidiarrheaTo investigate the calm> analgesic > antidiarrheal effects of SJ decoction , autonomic activities experiment >. hot-plate experiment and writhing reaction experiment antidiarrhea experiment induced by senna were used. SJ decoction could remarkably reduce the frequency of acetic acid-induced writhing ,delay the time of licking rear feet and could remarkably reduce the frequency of diarrhea .But the change of autonomic activities had no statistical significance. SJ decoction had affirmative analgesic and antidiarrheal effects, which could be the mechanism ofhealing IBS.4 Effect of SJ decoction on gastrointestinal motilityTo investigate the effect of SJ decoction on gastrointestinal motility, the change of intestinal propulsion ratio was observed in vivo.As well as the spontaneous contraction and the antagonistic contraction of intestinal stimulated by Ach , BaCh , 5-HT in vitro. The experiments showed: the intestinal propulsion ratio was remarkably reduced in SJ decoction groups. The spontaneous contraction and the antagonistic contraction of intestinal were dose-dependently inhibited after used the SJ decoction.The conclusion was :SJ decoction had restraining effect on the movement of intestinal tract, which was related to mechanism of action.
|
PDF Full Text Request