Pharmacodynamic Study And Mechanism Of Acorus Tatarinowii Schott And β-asarone On Preventing And Curing Acute Myocardial Infarction

Acute myocardial infarction(AMI) is a kind of coronary heart disease(CHD) which clinical harm is serious, especially happen arrhythmia, heart failure, cardiogenic shock and threat to life when the extent of myocardiolysis is large. People's living standard and life increase, the morbidity and mortality of AMI increase unceasingly, such as American with 1. 5 million people per year suffered from AMI, so the death number due to AMI is one fourth of total death number. Attack rate of AMI also goes up year by year, and it has become common and frequently encountered disease in our country. So it brings heavy burden to our society and family. How to prevent and cute AMI? How to prevent the serious complication? These questions become hot spots. Near these years, Western medicine has a great development on treating AMI, but there are still many questions unsolved. Traditional Chinese medicine has some superiority on treating AMI, which can improve patients' clinical symptom, raise viability and survival rate, improve lingering effects, and so on. However , the existent Chinese drugs preparation has. some distance from clinic, so that it is very important to manufacture more and better Chinese drugs preparation which absorb fastly, sume conveniently, effect quickly and visibly.Background:The medical using part(SCP) of Acorus Tatarinowii Schott (the plant is belong to Araceae )is its dry rhizome. SCP is used generally to treat cardiovascular and cerebrovascular disease. Up to now, There have been many and penetrating studies on SCP acting on central nervous system, but there are fewer studies on SCP acting on cardiovascular system and myocardial infarction. Near these years, my unity found that SCP has the following actions: adjustingexcitability of CNS, enhancing the permeability of blood encephalon, lightening cerebral edema on model rats which have caught cerebral paralysis, lightening apoptosis of cranial nerves, relaxing blood vessels, resisting platelet aggregation activity, improving learning ability and dysmnesia. The most effective parts are volatile oil and f3-asarol. There are lots of common physiological and pathological foundation between cardiovascular disease and cerebrovascular disease, especially on arteriosclerosis, injury of blood vessel and thrombosis. Thus, we infer that SCP might be acting well on myocardial infarction. So author tentatively investigate the effect of volatile oil of SCP and f3 -asarone which resist myocardial ischemia. The result discovered that they can lighten the damage of myocardial cell significant, we select "Pharmacodynamic Study and Mechanism of SCP and 3 -asarone on Preventing and Curing Acute Myocardial Infarction" for research to identify its action and approach its mechanism, our point is to offer experiment foundation for manufacturing the new medicine.Aim: Though Studying on volatile oil of SCP and P-asarone effect on acute myocardial infarction a/id approaching its acting mechanism initially to offer scientific experimental foundation for manufacturing the new traditional Chinese medicine on prevention and curing acute myocardial infarction.Methods: Through experiments of atherosclerosis, myocardial ischemia, thrombosis, hyperperviscous blood, hypoxia, clotting time, lyofibrin in vitro, and using the following indexes: blood-fat(CHOL, TG, HDL-C, LDL-C), ET, NO, CGRP, 5-HT, NE, EPI, D-dimer, Fg, PT, APTT, hemorheology, platelet aggregation, weight of thrombus, clotting time of mouse, the weight of plasma fibrin, spot of atherosclerosis, morphology of muscular tissue, and so on, to generously inspect volatile oil of SCP and P-asarone on protecting myocardial infarction, and approach its mechanism of action.Results: ?Volatile oil of SCP and P -asarone can cut down the blood-fat and ET, and raise the content of NO of hyperlipemia rats;?Volatile oil of SCP and P -asarone can cut down the levels of ET, CGRP and NE, and raise the content, of NO of myocardial ischemia rats, and relieve necrosis of myocardial ischemia;?Volatile oil of SCP and 3 -asarone can lower the weight of phlebothrombosis in rats, and extend PT and APTT of blood plasma in rats;?Volatile oil of SCP and P -asarone can improve hemorheology in the rats which have got hyperviscous, especially cut down viscositys of whole blood and blood plasmaobviously;(5)Vlatile oil of SCP and P -asarone can reinforce the mice' s anoxic ability;?Volatile oil of SCP and 3 -asarone can prolong mice' s clotting time obviously;?Volatile oil of SCP and P -asarone can solve plasma fibrin;(8)The effect volatile oil of SCP gets close 0-asarone, so that 3-asarone is major active component of volatile oil of SCP on preventing and curing acute myocardial infarction.Conclusion: Results indicate that volatile oil of SCP and 3-asarone can drop blood-fat, resist myocardial ischemia, refrain thrombosis, anticoagulation, lyofibrin, which mechanism of action maybe relate to the following factors: regulate the levels of ET, CGRP, NO, NE, and cut down myocardial consumption of oxygen, extend PT and APTT, improve blood glutinousness. It prompts it may have satisfactory therapeutic efficacy in clinical acute myocardial infarction.