| Objective: D1S80 and vWA were sorted out and verified the possibility of application in identification of mixed-up formalin-fixed, paraffin-embedded tissue. Methods :①60 cases of formalin-fixed ,paraffin-embedded colon cancer tissue specimens and their corresponding peripheral blood were collected, blood DNA and paraffin-embedded tissue DNA were extracted by improved phenol-chloroform method and improved proteinase-digested phenol-chloroform method,respectively. Selected genes were amplified by standard polymerase chain reaction ,and the product was analyzed by agarose electrophoresis and polyacrylamide gel electrophoresis (PAGE).②All patients' tumour tissue specimens were incised into 3 pieces and were fixed in10% formalin for 12,36,and 60 hour respectively, subsequently these specimens were detected by PCR technique. During the DNA-extracted process, each specimen was divided into 2 groups and digested by proteinase K for 12 hours at 37℃and for 3 hours at 56℃respectively,and DNA obtained were detected by PCR technique.③Search for the optimum PCR effect by adjusting the PCR reaction system. Results:①D1S80 was amplified successfully from blood DNA in all cases (30/30 cases, 100%),but 0 from paraffin-embedded tissue DNA (0/10 cases), and the vWA locus was amplified from blood and tissue DNA in 90%(27/30 cases) and 46.7% (14/30 cases) respectively. The total positive amplification of the 2 loci at tissue specimens were 0(0/45 specimens) and 29.7% (46/155 specimens),respectively.②Longer fixation time don't influence amplification for vWA locus(P>0.05).During the DNA-extracted process, temperature and time of incubation with proteinase K...
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