| ObjectiveThe purpose of the study was to analyze the relationship between the vascular endothelial growth factor (VEGF) and the survival of flap in the rat back ,which through the radiotherapy. We discussed the problem from three aspect that the relation between quantity and effect, time and effect, times and effect, we injection the VEGF into rat flap panniculus carnosus directly .MethodsThis experimentation adopted many methods to examine the study results. Such as naked eye observe, pathological observe and analysis, perfuse Chinese ink in blood vessel technique, circulate fluorescence staining skill combine with photoshop picture color analysis, determine the amber acid doff hydrogen enzyme in flap.1. Establish the animal model: an ischemic rat flap model, in this model, Wistar rat, 200±20g, general anesthesia , a 3×5cm full thickness dorsal flap with the pedicle remaining attached at the posterior end was elevated in all rats. Them were given 60Go radiotherapy in the two ,fourth ,sixth day postoperatively. Rats were irradiated with 3 fractions of 4 Gy.2. Quantity and effect experiment: The aim of this study was to test for different amount VEGF how to effect the survival of flap. Wistar rats twenty five were randomized equally into five groups and received the following treatment : Group VEGF were given under dermal injection into the flap; Group Control receive normal saline. All rats were offered fluorescent light staining in the...
|
PDF Full Text Request