Expression Of CD4, CD8 And CD28 On T Cells In Peripheral Blood From Patients With Multiple Sclerosis

Multiple sclerosis is a demyelinating disease in white matter of centralnervous system,and its exact pathogenesy is not fully distinct. The patholoicalfeature is inflammatory infiltration ,demyelination and auxiliary fibers castedof patching in central nervous system. Immune reaction mediated byleukomonocyte especially T leukomonocyte directly at self myelin protein isimportant in pathogenesy of MS.CD4 and CD8 are both glycoprotein expressed on T cell, and they areimportant leukomonocyte subpopulation mark . Th1 and Th2 cytokineproduced by activated CD4 T leukomonocyte that they disequilibrium. CD8+Tcan excret cell cytokine and promote facil macrophage's activation. InvasionCD4 and CD8 are close to MS. In immune reaction,default of secondsignal(costimulatory signal) provided by costimulatory molecule of Tleukomonocyte will make T leukomonocyte lead to clonal anerge andapoptosis. CD28 is a important costimulatory molecules expressed on Tleukomonocyte ,and it has norientation function to activation of Tleukomonocyte. Promoting multiplicationof T cell ,activation, secreting IL-2,externalization anti-apoptosis factor of Bcl-2 and Bcl-xl.Study expressing change of T leukomonocyte and costimulatorymolecules in peripheral blood with MS, has direction significance to monitorthat patient's condition relapse or relief and understand relation with its Lesiondegree .The study detects CD4 and CD8 on T leukomonocyte andcostimulatory molecules CD28 in peripheral blood and utilize EDSSmeasuring scale grade disability of injury with MS;the aim at approaching therelation between CD4 and CD8 on T leukomonocyte and costimulatorymolecules CD28 in peripheral blood and relapsing or reliefing and understandrelation with its lesion degree ,to expect providing base on clinic diagnose andmonitoring treatment , estimating lesion degree and relapsing-reliefing.We choose 28 patients with MS whose onset duration from2005.3~2006.4 at the department of neurology in China Japan Union Hospitalof Jilin University, all patients suit the relapse-relief;diagnostic standard ofPoser, according to Kurtzke score ,graded adopt flow cytometry measure CD4and CD8 expression and costimulatory molecules CD28 in 25 patients withMS and 16 patients of control group, Observe its relation between CD4 andCD8 on T leukomonocyte and costimulatory molecules CD28 in peripheralblood and relapse or relief and understand relation with its lesion degree .Theresult shows,.the expression of CD4+ and CD4+/CD8+ ratio in peripheral bloodof MS in relapse are both super than MS in relief and control relief .theexpression of CD8+cell and CD28+cell are both infra than MS in relief andcontrol relief;the expression of CD4+ and CD4+/CD8+ ratio in peripheralblood of MS in relief are super than MS in relief and control relief .theexpression of CD8+cell and CD28+cell are both infra than MS in control relief;and comparison of CD4+CD28+ in three group have no stastistisdifference,expression of CD8+ of MS patients whose lesion degree isweight .are lower than MS patients whose lesion degree is midrange.CD8+CD4+/CD8+ratio and CD8+ of different MS patients have no stastistisdifference.The expression of CD4+ in peripheral blood of MS patients hascorrelation with CD28+.At present we think that MS is autoallergic disease that individual havinghereditary under certain foreign aid mediator mediated by T cell .According toits function and surface marker,T cell can divide four mainsubgroups ,induction T cell /helper T cell ,casualties cell, suppressor T cell anddelayd hypersensitivity T cell ,T leukomonocyte control disequilibrium oflattic and are relevant with MS ,the quantity orfunction of CD4+and CD8+changes,T leukomonocyte regulation areoverbalance,then leads to illness.ofplatelet activation state in patients with acute cerebral infarction, offer clinicalindicators, can help us appraise the medicine curative effect and monitor thepatient's condition, and then guide clinical strategy. We gauge relevantparameters of platelet and make correlation analysis with MS .At the sametime, observe the effects of Ozagrel, then make clinical approach foranti-platelet medicine usage, and then offer theoretical foundation fortreatment of acute cerebral infarction.They hints there are T leukomonocyteimmunological regulation disorder,helping cell function enhanced, suppressorcell function defect, reflecting CD4+ and CD8+ abnormally increase ordecrease in peripheral blood of MS,lead to the abnormally of CD4+/CD8+ratio,it is abnormal immunology foundation ,important for genesis anddevelopment of MS.CD4+ T cell can promote the generation and differentiation of B cell,of Tcell and other immunocell,and coordinate the interaction in immunocell,recentstudy show in pathogenesy of MS,activated CD4+ T cell can produce Th1 andparticipate genesis and development of MS, CD8+ T cells are helewglnouovaccine condition CD4+ and CD8+ of T cell subgroup keep inbariably ratio.They induce and restrict each other ,T cellular network produced by them areimportant for controlling immune reaction and keeping immune balance.In T cell activation ,unless costimulatory signal,T cell have not reactionand immune tolerance,CD28 is the most costimulatory signal activated by Tcell, whose nature liganal lies in B7 molecule family on antige presentingcell(APC)(including B7.1,B7.2,B7.3).Our study show CD4+T cell of MSpatients increased,followed that costimulatory molecules CD28+decreased ,which indicates costimulatory molecules CD28+ has importantfunction in CD4+T cell abnormally activation,thus the expression ofcostimulatory molecules CD28 may be a worthy and feasible clinical immunoindex that judges prognosis.The study utilize EDSS measuring scale grade disability of injury of MSpatients and according scores devided MS patients middle and heavydegree,compared and analyzed CD4+ ,CD8+ and costimulatory molecules ofdifferent lesion degree.our data indicates the expressin of CD8+ is concernedwith lesion serious degree of MS.the expression of CD4+ and CD28+ may beconcerned with repeated frequency of MS. Study expression and change of Tleukomonocyte and costimulatry molecules in peripheral of MS,the aim isprovide base for diagnosis,montoring therapy and estimate lesion degree andrelapse relief.Detect T leukomonocyte and costimulatory noninvasively flowcytometry provided conduced to understand deeply immunology mechanismof MS.thus using flow cytometry detect CD4,CD8 and costimulatorymolecules CD28 of MS.having potential application value in directing clinic.Itmay provide prognostic index for clinic MS patients to monitoring conditionrelapse relief.To sum up,detect the expression of CD4+,CD8+and costimatory moleculeCD28 ,use EDSS measuing scale grades disability of injury of MS.We canmake following conclusion:There are T leukomonocyte disorder in MSpatients including enhanced helping cell function and function defect ofhelping cell,CD4 and CD8 participate in pathogenesy of MS;CD28 play animportant role in the abnormal activation of CD4+T leukomonocytecostimutory molecules CD28 may participate in its pathogenesy;theexpression of CD8+ and CD4+/CD8+ ratio concern with lesion degree,theexpression of CD4+ and CD28+ concern with relapsing and relief. CD4,CD8and CD28 may be one of clinical indexs of monitoring MS patients ofcondition change.