| Objective To investigate the genes variation of apolipoprotein A5 influence on the patients with coronary artery disease (CAD) and lipid disorder, We wished to demonstrate whether apoA5 SNPs is a genetic marker for CAD, or a strong linkage disequilibrium were reflected between apoA5 SNPs and apoC3 (-482 C/T) which would be an evident for apoC3 as a genetic marker;as well as predominance of haplotype would effect genetic hereditation on CAD. The study also researched apoA5 and apoC3 SNPs effect on metabolism lipid, subsequently lead to CAD.Methods The study was investigated by case-control method, 195 cases of CAD vs 181 normal, all the subjects were unrelated Shandong han which matched age and gender. The gene variation of apolipoprotein series including apoA5 SNP3 (-113 IT >C), S19W(C56C>G) , SNP4 (-12238T>C) and apoC3 (-482C/T) were identified by polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP ) and DNA sequence. The parameters of plasma lipids were measured through biochemistry, as well as these sites of genes were combined to study on influence CAD. Results The frequency of the apoA5 SNP3 TC in patients with CAD was significantly higher than that of the control group(P<0.05), and this difference existed after adjustment for age, gender, levels of lipid and so on. The levels of TG and TC were significantly elevated in SNP4 TC (P <0.05), but there was no significant difference after adjusting the above risk factors. The influence of apoC3 (-482 C/T) on CAD disappeared after adjustment. The strong linkage disequilibrium were reflected between apoA5 SNP3 and apoC3 (-482 C/T) (P<0.05) in CAD group. The frequency of haplotype block of apoA5 *3- apoC3-482T in CAD group was lower than that in control group. In CAD patients, the levels of triglyceride (TG) were significantly elevated in apoA5 SNP3 TC compared to two other genotypes, as well as were increased in apoC3 -482 CT compared to genotypes of CC and TT;either of them was independent factor for TG levels. Conclusions ApoA5 SNP3 (-1131T>C) maybe a genetic marker for CAD;The association is found between SNP4 polymorphism and CAD( P <0.05). The incidence ofCAD may be increased by the lipid disorder for the variation of SNP4. In CAD patients, the levels of TG were significantly elevated in apoA5 SNP3 TC compared to two other genotype, as well as were increased in apoC3 -482 CT compared to genotype of CC and TT;either of them was independent factor for TG levels;the variation of either apoA5 SNP3 or apoC3 -482 influence plasma TG levels. Haplotype block apoA5 * 3-apoC3-482T effect genetic susceptibility on CAD. |
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