Quantitative Study Of Cytokeratin Expressions At Deepness Resection Margins Of Glottic Cancer

Objectives 60 cases with glottic cancer were performed with pathology observation and study of Cytokeratin expressions. Exploring the safe resection deepness margins of glottic cancer can provide evidence for clinical treatment.Methods 60 cases with glottic cancer and completed clinical information were collected, all having no radiotherapy and chemiotherapy before operation. Different laryngectomy and partial laryngectomy were operated according to TNM staging. Deepness resection margins were signatured during operation. After having been coped with 10% neutral Fuermalin, the samples with signa were cut into block per 3mm distances continuously. Choose the biggest with tumor tissue and cut it into slices per 5um distances continuously. One of sixty slices was HE and immunohistochemistry stained. Glottic cancer tissue with different staging and deepness resection margins with different distances were performed with pathology observation and study of Cytoleratin expression.Results For glottic cancer with T1 staging, there was a significant difference of Cytokeratin expressions between tumor and the resection margins at 2,3,5mm, p<0.05, yet there was no significant difference between 2,3,5mm,p>0.05. For glottic cancer with T2 and T3 staging .there was no significant difference of Cytokeratin expressions between tumor and 2mm, 3 and 5mm, p>0.05, but there was a significant difference between tumor and 3,5mm, 2 and 3,5mm. For glottic cancer with T4 staging ,there was no significant difference of Cytoketin expressions between tumor and 2, 3mm, 2 and 3mm, p>0.05, but there was a significant difference between tumor and 5mm, 2,3 and 5mm, p< 0.05.Conclusions Different staging .different the deepness resection margins of glottic cancer. The safe deepness resection margins of glottic cancer with T1 staging is 2mm, T2 T3 3mm, and T4 staging is 5mm at least.