The Observation Of Serum IL-12 And IL-10 In Different Stage Of Children With AITD

Objective:Hashimoto thyroiditis(HT) and Graves' disease(GD) are common chronic autoimmune disease(AITD). It is generally accepted that the change of Thl/Th2 cells and Thl/Th2 cytokines balance is implicated in the pathogenesis of AITD. We studied the level of interleukin-lO(IL-lO) and interleukin-12(IL-12) in serum of patients with AITD in different stage of disease, explored the relation between IL—10, IL-12 and thyroid function, antibody et al and the effect of these two cytokines on AITD, which may provide a new approach to treat AITD. Methods:The serum IL-10 and IL-12 concentration was measured in 77 HT children, 39 GD children and 31 normal subjects by ELISA and we compared the level of IL-10 and IL-12 in HT and GD in different stage respectively. We also measured free thyroid function(FT4, FT3, sTSH) and thyroid auto-antibody(TGAb, TMAb, TRAb, TSI) of all subjects, analysed the correlativity of IL-10, IL-12 and other parameters. Results:1. The serum TGAb, TMAb, TRAb and TSI concentration of HT and GD subjects were higher than normal subjects (P<0. 05).2. The serum IL-12 concentration of HT subjects(271. 41 ± 143. 45pg/ml) were higher than normal subjects (175. 41+63. 03pg/ml, P=0. 002). That of the new diagnosis subjects were higher than that of these accepting treatment and normal subjects. Among these accepting treatment, that ofhypothyroidisms were higher than that of euthyrodisms and normal subjects.The serum IL-10 concentration of GD(9. 52±6. 84pg/ml) were lower than that of normal subjects(21. 80±5. 37pg/ml, P=0.001)\ That of the three groups were all lower than normal subjects. That of the new diagnosis subjects were lower than these accepting treatment. Among these accepting treatment, that of the hypothyroidisms were lower than that of the euthyrodisms.3. The serum IL-12 (209. 21 ± 118. 65pg/ml, P = 0. 000) were higher than normal subjects. That of the new diagnosis subjects were higher than these accepting treatment and normal subjects. Among these accepting treatment, that of the hyperthyroidisms were higher than the euthyrodisms.The serum IL-10 concentration of GD subjects (24. 19±7. 59pg/ml, P = 0.037) were higher than normal sub jects. That of the new diagnosis subjects were higher than these accepting treatment and normal sub jects. Among these accepting treatment, that of the hyperthyroidisms were higher than the euthyrodisms.4. Among HT subjects, the serum IL-12 was no significantly correlation with age, TGAb, TMAb, TRAb and TSI, and was negatively significantly correlation with FT4, FT3(r= - 0. 763, P = 0. 000 and r= - 0. 638, P=0. 000 respectively) and positively correlation with sTSH(r = 0. 514 , P = 0.000). The serum IL-10 was no significantly correlation with age, TGAb, TMAb, TRAb and TSI, and was positively significantly correlation with FT4, FT3(r=0. 452, P=0. 000 and r=0. 423, P=0. 000 respectively) andnegatively correlation with sTSH(r= - 0. 279, P=0.014).5. Among GD subjects, the serum IL-12 was no significantly correlation with age, FT4, FT3, TGAb, TMAb, TRAb and TSI, and was negatively significantly correlation with sTSH(r= - 0. 309,P<0. 05). The serum IL-10 was no significantly correlation with age, sTSH, TGAb, TMAb, TRAb and TSI,and was positively significantly correlation with FT4,FT3(r = 0. 350, P=0. 029 and r=0. 334, P=0. 037respectively).6. Logistic regression analysis showed FT4,sTSH, TMAb and TGAb were obvious factors that affected IL-12 in HT. The equation was IL-12 = 594.91-28. 72FT4+1.289sTSH+357.097TMAb-198.581TGAb. TMAb was obvious factor that affected IL-10 in HT. The equation was IL-10=-2.17-23. 562 TMAb.Logistic regression analysis showed sTSH and TSI were obvious factors that affected IL-12 in GD. The equation was IL-12 = 235.91-35.24sTSH+26.492TSI. TRAb and TSI were obvious factors that affected IL-10 in GD. The equation was IL-10=9. 226+10. 525TRAb+2. 567TSK Conclusions:1. The serum TGAb, TMAb, TRAb and TSI concentration of HT and GD subjects were higher than normal subjects.It's confirmed the idea that except thyroid dysfunction, thyroid auto-antibodies production was involved in AITD. These antibodies impacted on its evolvement, and could be a sign of diagnosis, estimating prognosis and stopping dosing.2. The serum IL-12 concentration of HT subjects were higher than normal subjects. Te new diagnosis subjects were higher than these accepting treatment and normalsubjects. Among these accepting treatment, thehypothyroidisms were higher than the euthyrodisms and normal subjects. The serum IL-10 concentration were lower than that of normal subjects. The three groups were all lower than normal subjects. The new diagnosis subjects were lower than these accepting treatment. Among these accepting treatment, the hypothyroidisms were lower than the euthyrodisms. It' s confirmed the idea that IL-12 is concerned with disease progress. Contrarily, IL-10 has protective effect on HT.3. The serum IL-12 concentration of GD subjects were higher than normal subjects. The new diagnosis subjects were higher than these accepting treatment and normal subjects. And the hyperthyroidisms were higher than the euthyrodisms. The serum IL-10 concentration of GD subjects were higher than normal subjects. The new diagnosis subjects were higher than these accepting treatment and normal subjects. Among these accepting treatment, the hyperthyroidisms were higher than the euthyrodisms. It suggests that both of these two cytokines are involved in this disease.4. Among HT subjects,the serum IL-12 was negatively significantly correlation with FT4, FT3 and positively correlation with sTSH, the serum IL-10 was positively significantly correlation with FT4, FT3 and negatively correlation with sTSH. It sugests that IL-12 can reflect the severity of HT. While IL-10 can prevent HT from deteriorating, reduce thyroid destruction and can be a sign of disease comeback.5. Among GD subjects, the serum IL-12 was negatively significantly correlation with sTSH, the serum IL-10 was positively significantly correlation with FT4 and FT3. The serum IL-12 concentration was higher in hyperthyroidisms, but its degree was lower than in HT subjects. SerumIL-10 concentration increased obviously. Both IL-12 and IL-10 had effect on GD and the latter was more important, which can be a sign of state of the disease.